Human intestinal anion exchanger isoforms: expression, distribution, and membrane localization
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چکیده
منابع مشابه
Molecular cloning, expression, and chromosomal localization of two isoforms of the AE3 anion exchanger from human heart.
Cl-/HCO3- exchange contributes to regulation of pHi and [Cl-] in cardiac muscle, with possible effects on excitability and contractility. We have isolated human heart cDNAs, which encode two isoforms of the anion exchanger AE3. These clones share long portions of common sequence but have different 5' ends encoding distinct amino-terminal amino acid sequences. The longer AE3 polypeptide of 1232 ...
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Previously, we isolated the human AE2 (SLC4A2) gene, a member of the sodium-independent anion exchanger family. Rat ortholog of this gene was reported to drive alternative transcription yielding N-terminal variants of the AE2a message. We thus analyzed the human AE2 gene in this regard. Using HepG2 cells, two alternative first exons, each splicing to exon 3 in alternative transcripts, were foun...
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Recent studies from our laboratory and others have demonstrated the involvement of monocarboxylate transporter (MCT)1 in the luminal uptake of short-chain fatty acids (SCFAs) in the human intestine. Functional studies from our laboratory previously demonstrated kinetically distinct SCFA transporters on the apical and basolateral membranes of human colonocytes. Although apical SCFA uptake is med...
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AIMS To determine the distribution of the alpha1 to alpha6 chains of type IV collagen in Bruch's membrane of the human posterior pole. METHODS Cryosections (10 micro m) from 18 human eyes (20 months to 83 years old) were acid treated, blocked with 10% normal goat serum, incubated for 1 hour with monoclonal antibodies against type IV collagen isoform specific peptides at 1:75 dilution, and vis...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Biomembranes
سال: 2001
ISSN: 0005-2736
DOI: 10.1016/s0005-2736(00)00366-7